Cellulose Acetate Paper Electrophoresis

Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample.

Cellulose acetate paper electrophoresis.

Cellulose acetate is the acetate ester of cellulose it was first prepared in 1865. It is also used as a synthetic fiber in the manufacture of cigarette filters and playing cards in photographic film cellulose acetate replaced nitrate film in the 1950s being far. After this immerse both the ends of cellulose acetate filter paper in a buffer solution. The cellulose acetate system is the ideal tank for both standard and wet cellulose acetate electrophoresis.

Electrophoresis on cellulose acetate paper agarose and polyacrylamide has been used as a means of separating the lipoprotein classes and indeed the separation pattern on paper electrophoresis was the basis of the fredrickson classification of lipid disorders. Provides a good background or staining glycoprotein application. It is a modified version of paper electrophore sis developed by kohn in 1958. Cellulose acetate electrophoresis the helena electrophoresis system helena laboratories beaumont tx usa was used to electrophorese hemolysates on titan iii cellulose acetate plates at 350 v for 25 min in an alkaline buffer.

The electrophoresis on cellulose acetate membrane is most widely used because of its simplicity and is without the use of any sophisticated instrument other than electrophoresis apparatus and the cellulose acetate strip. On cathode side of midline current 0 4 ma cm width of paper. Cellulose acetate electrophoresis 23. Cellulose acetate electrophoresis is an important technique in clinical diagnostics.

Electrophoresis is typically performed at ph 8 6 using cellulose acetate as the support medium. At this ph the overall hemoglobin molecule is negatively charged and when placed in an electric field will move towards the positive terminal anode. First the cellulose acetate filter paper moistens with the buffer solution. It involves the following steps.

In this type of electrophoresis bacteriological acetate mem brane filters are taken in place of regular chro matography paper. It gives sharper bands. Cellulose acetate is used as a film base in photography as a component in some coatings and as a frame material for eyeglasses. 12x2 5 cm sample applied from micropipette or capillary along a line about 1 cm.

Widely used in analysis of clinical and biological protein sample albumin and globulins. After staining strips are made transparent by soaking in whitmore s oil or liquid paraffin and then suitable for transmittance densitometry of the protein bands. Alternative to paper electrophoresis. Cellulose acetate electrophoresis it contains 2 3 acetyl groups per glucose unit and its adsorption capacity is less than that of paper.