Surface areas of cell culture vessels the following are the surface areas of various tissue culture dishes or plates and their relative size to a 35 mm dish. Total flask area cm2 cell yield ml flask volume ml 25 cm2 25 2 5 x 106 5 7 5 70 rectangular 75 cm2 75 7 5 x 106 15 22 5 265 u shaped 150 cm2 150 1 5 x 107 30 45 377 u shaped 175 cm2 175 1 75 x 107 35 52 5 513 u shaped 225 cm2 225 2 25 x 107 45 67 5 1 006 traditional. Recommended growth average medium volume approx.
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