Cdk2 Antibody Cell Signaling

Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Cdk2 antibody cell signaling.

Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412. An antibody shouldn t be one of the variables in your experiment. It does not cross react with other phosphorylated cyclin dependent kinases. Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3.

Green signal from target ab32147 observed at 34 kda lanes 3 and 4. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor. Lane 1 is 10 input. Antibodies are purified by protein a and peptide affinity chromatography.

Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160. To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs. Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6. The antibody weakly cross reacts with cdc2 phosphorylated at thr161.

Western blot analysis was performed using cdk12 antibody. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Mix well then add 0 1 g bsa mix. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2. Find out why customers rank cst highest for antibody specificity and sensitivity. Merged red and green signal.

Lanes 1 3 and 5. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Cdk2 antibodies cdk2 antibodies p33cdk2 associates with cyclin a in human cells. Cdk2 knockout hap1 cell lysate 20 µg lanes 1 and 2.

Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Wild type hap1 cell lysate 20 µg lanes 2 4 and 6. Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used.

Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen. Recommended fluorochrome conjugated anti rabbit secondary antibodies.